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Original Article
- Proteomic Analysis of Intracellular and Membrane Proteins From Voriconazole-Resistant
Candida glabrata - Jae Il Yoo, Hwa Su Kim, Chi Won Choi, Jung Sik Yoo, Jae Yon Yu, Yeong Seon Lee
- Osong Public Health Res Perspect. 2013;4(6):293-300. Published online December 31, 2013
- DOI: https://doi.org/10.1016/j.phrp.2013.10.001
- 3,077 View
- 21 Download
- 3 Crossref
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Abstract
PDF - Objectives
The proteomic analysis of voriconazole resistant Candida glabrata strain has not yet been investigated. In this study, differentially expressed proteins of intracellular and membrane fraction from voriconazole-susceptible, susceptible dose-dependent (S-DD), resistant C. glabrata strains were compared with each other and several proteins were identified.
Methods
The proteins of intracellular and membrane were isolated by disrupting cells with glass bead and centrifugation from voriconazole susceptible, S-DD, and resistant C. glabrata strains. The abundance of expressed proteins was compared using two-dimensional sodium dodecyl sulfate polyacrylamide gel electrophoresis and proteins showing continuous twofold or more increase or reduction of expression in resistant strains compared to susceptible and S-DD strain were analyzed by liquid chromatography/mass spectrometry-mass spectrometry method.
Results
Of 34 intracellular proteins, 15 proteins showed expression increase or reduction (twofold or more). The identified proteins included regulation, energy production, carbohydrate transport, amino acid transport, and various metabolism related proteins. The increase of expression of heat shock protein 70 was found. Among membrane proteins, 12, 31 proteins showed expression increase or decrease in the order of susceptible, S-DD, and resistant strains. This expression included carbohydrate metabolism, amino acid synthesis, and response to stress-related proteins. In membrane fractions, the change of expression of 10 heat shock proteins was observed, and 9 heat shock protein 70 (Hsp70) showed the reduction of expression.
Conclusion
The expression of Hsp70 protein in membrane fraction is related to voriconazole resistant C. glabrata strains. -
Citations
Citations to this article as recorded by
- What ‘Omics can tell us about antifungal adaptation
Gabriela Fior Ribeiro, Eszter Denes, Helen Heaney, Delma S Childers
FEMS Yeast Research.2022;[Epub] CrossRef - Effects of antifungal agents on the fungal proteome: informing on mechanisms of sensitivity and resistance
Rebecca A. Owens, Sean Doyle
Expert Review of Proteomics.2021; 18(3): 185. CrossRef - HPLC-MS identification and expression of Candida drug-resistance proteins from African HIV-infected patients
Pedro M D S Abrantes, Randall Fisher, Patrick J D Bouic, Carole P McArthur, Burtram C Fielding, Charlene W J Africa
AIMS Microbiology.2021; 7(3): 320. CrossRef
- What ‘Omics can tell us about antifungal adaptation
Article
- Proteomic Analysis of Cellular and Membrane Proteins in Fluconazole-Resistant
Candida glabrata - Jae Il Yoo, Chi Won Choi, Hwa Su Kim, Jung Sik Yoo, Young Hee Jeong, Yeong Seon Lee
- Osong Public Health Res Perspect. 2012;3(2):74-78. Published online June 30, 2012
- DOI: https://doi.org/10.1016/j.phrp.2012.04.001
- 2,758 View
- 15 Download
- 6 Crossref
-
Abstract
PDF
- Objectives
Candida glabrata is one of the most common causes of Candida bloodstream infections worldwide. Some isolates of C glabrata may be intermediately resistant to azoles, with some strains developing resistance during therapy or prophylaxis with fluconazole. In this study, we used a proteomic approach to identify differentially expressed proteins between fluconazoleresistant and -susceptible strains.
Methods
Membrane and cellular proteins were extracted from fluconazolesusceptible and fluconazole-resistant C glabrata strains. Differentially expressed proteins were compared using two-dimensional sodium dodecyl sulfate polyacrylamide gel electrophoresis. Proteins with >1.5-fold difference in expression were identified by liquid chromatography tandem mass spectrometry (LC-MS/MS).
Results
A total of 65 proteins were differentially expressed in the cellular and membrane fractions. Among the 39 cellular proteins, 11 were upregulated and 28 were downregulated in fluconazole-resistant strains in comparison with fluconazole-susceptible strains. In the membrane fraction, a total of 26 proteins were found, of which 19 were upregulated and seven were downregulated. A total of 31 proteins were identified by LC-MS/MS that are involved in glycolysis, carbohydrate transport, energy transfer, and other metabolic pathways. Heat shock proteins were identified in various spots.
Conclusion
Heat shock and stress response proteins were upregulated in the membrane fraction of the fluconazole-resistant C glabrata strain. Compared with susceptible strains, fluconazole-resistant strains showed increased expression of membrane proteins and decreased expression of cellular proteins. -
Citations
Citations to this article as recorded by- What ‘Omics can tell us about antifungal adaptation
Gabriela Fior Ribeiro, Eszter Denes, Helen Heaney, Delma S Childers
FEMS Yeast Research.2022;[Epub] CrossRef - Effects of antifungal agents on the fungal proteome: informing on mechanisms of sensitivity and resistance
Rebecca A. Owens, Sean Doyle
Expert Review of Proteomics.2021; 18(3): 185. CrossRef - HPLC-MS identification and expression of Candida drug-resistance proteins from African HIV-infected patients
Pedro M D S Abrantes, Randall Fisher, Patrick J D Bouic, Carole P McArthur, Burtram C Fielding, Charlene W J Africa
AIMS Microbiology.2021; 7(3): 320. CrossRef - Transcriptional responses of Candida glabrata biofilm cells to fluconazole are modulated by the carbon source
Rosana Alves, Stavroula L. Kastora, Alexandra Gomes-Gonçalves, Nuno Azevedo, Célia F. Rodrigues, Sónia Silva, Liesbeth Demuyser, Patrick Van Dijck, Margarida Casal, Alistair J. P. Brown, Mariana Henriques, Sandra Paiva
npj Biofilms and Microbiomes.2020;[Epub] CrossRef - Proteomics Analysis of Candida albicans dnm1 Haploid Mutant Unraveled the Association between Mitochondrial Fission and Antifungal Susceptibility
Thuyen Truong, Guisheng Zeng, Teck Kwang Lim, Tong Cao, Li Mei Pang, Yew Mun Lee, Qingsong Lin, Yue Wang, Chaminda Jayampath Seneviratne
PROTEOMICS.2020;[Epub] CrossRef - Membrane Proteome-Wide Response to the Antifungal Drug Clotrimazole in Candida glabrata: Role of the Transcription Factor CgPdr1 and the Drug:H+ Antiporters CgTpo1_1 and CgTpo1_2
Pedro Pais, Catarina Costa, Carla Pires, Kiminori Shimizu, Hiroji Chibana, Miguel C. Teixeira
Molecular & Cellular Proteomics.2016; 15(1): 57. CrossRef
- What ‘Omics can tell us about antifungal adaptation
Original Article
- Gene Expression and Identification Related to Fluconazole Resistance of
Candida glabrata Strains - Jae Il Yoo, Chi Won Choi, Kyeong Min Lee, Yeong Seon Lee
- Osong Public Health Res Perspect. 2010;1(1):36-41. Published online December 31, 2010
- DOI: https://doi.org/10.1016/j.phrp.2010.12.009
- 2,775 View
- 16 Download
- 9 Crossref
-
Abstract
PDF
- Objectives
Candida glabrata has become one of the most common causes of Candida bloodstream infections worldwide. Some strains of C. glabrata may be intermediately resistant to all azoles. The several possible mechanisms of azole resistance have been reported previously, but the exact resistant mechanism is not clear. In this study, we identified differentially expressed genes (DEGs) of fluconazole-resistant C. glabrata and compared the gene expression of fluconazole-resistant strains with that of fluconazole-susceptible strains to identify gene corresponding to fluconazole resistance.
Methods
Using antifungal susceptibility test, several C. glabrata strains were selected and used for further study. The expression of CgCDR1 and CgCDR2 genes was investigated by slot hybridization against fluconazole-susceptible, -resistant, and resistant-induced strains. In addition, ERG3 and ERG11 genes were sequenced to analyze DNA base substitution. DEGs were identified by reverse transcription-polymerase chain reaction using DEG kit composed of 120 random primers.
Results
In slot hybridization, CgCDR1 gene was expressed more than CgCDR2 gene in resistant strains. Though base substitution of ERG11 and ERG3 genes was observed in several base sequences, just one amino acid change was identified in resistant strain. In the results of reverse transcription-polymerase chain reaction, 44 genes were upregulated and 34 genes were downregulated. Among them, adenosine triphosphate-binding cassette transporter-related genes, fatty acid desaturase, lyase, and hypothetical protein genes were upregulated and aldehyde dehydrogenase, oxidoreductase, and prohibitin-like protein genes were downregulated. Other DEGs were also identified.
Conclusion
This study showed that CgCDR1 gene was more closely related to fluconazole resistance of C. glabrata than CgCDR2 gene. In addition, several other genes related with fluconazole resistance of C. glabrata were identified. -
Citations
Citations to this article as recorded by- Molecular Mechanisms Associated with Antifungal Resistance in Pathogenic Candida Species
Karolina M. Czajka, Krishnan Venkataraman, Danielle Brabant-Kirwan, Stacey A. Santi, Chris Verschoor, Vasu D. Appanna, Ravi Singh, Deborah P. Saunders, Sujeenthar Tharmalingam
Cells.2023; 12(22): 2655. CrossRef - Candida glabrata: Pathogenicity and Resistance Mechanisms for Adaptation and Survival
Yahaya Hassan, Shu Yih Chew, Leslie Thian Lung Than
Journal of Fungi.2021; 7(8): 667. CrossRef - Candidiasis and Mechanisms of Antifungal Resistance
Somanon Bhattacharya, Sutthichai Sae-Tia, Bettina C. Fries
Antibiotics.2020; 9(6): 312. CrossRef -
A Transcriptomics Approach To Unveiling the Mechanisms of
In Vitro
Evolution towards Fluconazole Resistance of a
Candida glabrata
Clinical Isolate
Mafalda Cavalheiro, Catarina Costa, Ana Silva-Dias, Isabel M. Miranda, Can Wang, Pedro Pais, Sandra N. Pinto, Dalila Mil-Homens, Michiyo Sato-Okamoto, Azusa Takahashi-Nakaguchi, Raquel M. Silva, Nuno P. Mira, Arsénio M. Fialho, Hiroji Chibana, Acácio G. R
Antimicrobial Agents and Chemotherapy.2019;[Epub] CrossRef - Clonal Spread of Candida glabrata Bloodstream Isolates and Fluconazole Resistance Affected by Prolonged Exposure: a 12-Year Single-Center Study in Belgium
Berdieke Goemaere, Katrien Lagrou, Isabel Spriet, Marijke Hendrickx, Pierre Becker
Antimicrobial Agents and Chemotherapy.2018;[Epub] CrossRef - Candida antifungal drug resistance in sub-Saharan African populations: A systematic review
Charlene Wilma Joyce Africa, Pedro Miguel dos Santos Abrantes
F1000Research.2017; 5: 2832. CrossRef - Expression Patterns of ABC Transporter Genes in Fluconazole-Resistant Candida glabrata
Atefeh Abdollahi Gohar, Hamid Badali, Tahereh Shokohi, Mojtaba Nabili, Nasrin Amirrajab, Maryam Moazeni
Mycopathologia.2017; 182(3-4): 273. CrossRef - Glabridin induces overexpression of two major apoptotic genes, MCA1 and NUC1 , in Candida albicans
Mojtaba Nabili, Maryam Moazeni, Mohammad Taghi Hedayati, Parisa Aryamlo, Atefeh Abdollahi Gohar, Seyed Mehdi Madani, Hamed Fathi
Journal of Global Antimicrobial Resistance.2017; 11: 52. CrossRef - Candida antifungal drug resistance in sub-Saharan African populations: A systematic review
Charlene Wilma Joyce Africa, Pedro Miguel dos Santos Abrantes
F1000Research.2016; 5: 2832. CrossRef
- Molecular Mechanisms Associated with Antifungal Resistance in Pathogenic Candida Species
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